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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
21/02/2014 |
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Actualizado : |
05/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FRAGA, M.; FERNÁNDEZ, S.; CAJARVILLE, C.; MARTÍNEZ, M.; ABIN-CARRIQUIRY, J.A.; ZUNINO, P. |
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Afiliación : |
MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Departamento de Microbiología Instituto de Investigaciones Biológicas Clemente Estable Montevideo Uruguay. |
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Título : |
In vitro modulation of rumen microbiota and fermentation by native microorganisms isolated from the rumen of a fed-exclusively-on-pasture bovine. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Annals of Microbiology, v. 65 n. 4, p. 2355-2362, 2015. |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 6 January 2015 /Accepted: 17 March 2015. |
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Contenido : |
Abstract:
In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by several strains. Also, the abundances of Lachnospiraceae, Veillonellaceae, Rikenellaceae and Succinivibrionaceae were affected by strain supplementation. Modulation of fermentation by selected ruminal native bacteria was achieved, probably enhancing the fermentation of non-soluble carbohydrates. This study represents a new approach in the knowledge related to the use of probiotics in ruminants. MenosAbstract:
In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by seve... Presentar Todo |
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Palabras claves : |
FERMENTATION MODULATION; IN VITRO GAS PRODUCTION; MICROBIOTA RUMINAL; NATIVE RUMINAL MICROORGANISMS; PROBIÓTICOS; PROBIOTICS; RUMEN MICROBIOTA. |
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Thesagro : |
PRODUCCIÓN ANIMAL. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02838naa a2200289 a 4500
001 1049916
005 2019-11-05
008 2015 bl uuuu u00u1 u #d
100 1 $aFRAGA, M.
245 $aIn vitro modulation of rumen microbiota and fermentation by native microorganisms isolated from the rumen of a fed-exclusively-on-pasture bovine.
260 $c2015
500 $aArticle history: Received: 6 January 2015 /Accepted: 17 March 2015.
520 $aAbstract: In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by several strains. Also, the abundances of Lachnospiraceae, Veillonellaceae, Rikenellaceae and Succinivibrionaceae were affected by strain supplementation. Modulation of fermentation by selected ruminal native bacteria was achieved, probably enhancing the fermentation of non-soluble carbohydrates. This study represents a new approach in the knowledge related to the use of probiotics in ruminants.
650 $aPRODUCCIÓN ANIMAL
653 $aFERMENTATION MODULATION
653 $aIN VITRO GAS PRODUCTION
653 $aMICROBIOTA RUMINAL
653 $aNATIVE RUMINAL MICROORGANISMS
653 $aPROBIÓTICOS
653 $aPROBIOTICS
653 $aRUMEN MICROBIOTA
700 1 $aFERNÁNDEZ, S.
700 1 $aCAJARVILLE, C.
700 1 $aMARTÍNEZ, M.
700 1 $aABIN-CARRIQUIRY, J.A.
700 1 $aZUNINO, P.
773 $tAnnals of Microbiology$gv. 65 n. 4, p. 2355-2362, 2015.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha actual : |
08/01/2015 |
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Actualizado : |
20/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
AGUERRE, V.; ALBICETTE, M.M.; RUGGIA, A.; SCARLATO, S.; BLUMETTO, O.; BORTAGARAY, I.; CARDOZO, G.; CASTAGNA, A.; CLARA, P.; GARCIA, F.; GILSANZ, J.C.; LEONI, C.; QUINTANS, G.; SCARLATO, M.; SILVERA, M.; TISCORNIA, G.; VILARO, F.; ALBIN, A. |
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Afiliación : |
MARIA VERONICA AGUERRE ANTIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA MARTA ALBICETTE BASTRERI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA PAOLA RUGGIA CHIESA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANTIAGO SCARLATO GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OSCAR RICARDO BLUMETTO VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ISABEL BORTAGARAY, Técnico participante del Proyecto; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRES CASTAGNA DU PRE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO DANIEL CLARA PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELIPE LUIS GARCIA OLASO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN CARLOS GILSANZ MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GRACIELA QUINTANS ILARIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIANA SCARLATO GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIANA SILVERA ORREGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUADALUPE TISCORNIA TOSAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALFREDO SANTIAGO ALBIN FERREIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
El desafío para la ganadería familiar: ¿cómo aumentar producción e ingresos conservando el medio ambiente?. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Revista INIA Uruguay, 2014, No.39, p.75-78. |
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Serie : |
(Revista INIA; 39) |
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ISSN : |
1510-9011 |
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Idioma : |
Español |
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Contenido : |
El objetivo es adaptar el enfoque de co-innovación en sistemas ganaderos familiares criadores sobre campo natural, a través de su aplicación en predios, evaluando
el impacto de la implementación de cambios estratégicos (re-diseño) en la sustentabilidad de esos predios, procurando compartir los resultados obtenidos con
otros actores a nivel regional. |
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Thesagro : |
DESARROLLO SOSTENIBLE; INVESTIGACIÓN AGRARIA; PRODUCCION FAMILIAR; SOCIOLOGIA RURAL. |
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Asunto categoría : |
E10 Economía y políticas agrícolas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3916/1/Rev.INIA-2014-No39-p.75-78.pdf
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Marc : |
LEADER 01424naa a2200397 a 4500
001 1051983
005 2020-02-20
008 2014 bl uuuu u00u1 u #d
022 $a1510-9011
100 1 $aAGUERRE, V.
245 $aEl desafío para la ganadería familiar$b¿cómo aumentar producción e ingresos conservando el medio ambiente?.
260 $c2014
490 $a(Revista INIA; 39)
520 $aEl objetivo es adaptar el enfoque de co-innovación en sistemas ganaderos familiares criadores sobre campo natural, a través de su aplicación en predios, evaluando el impacto de la implementación de cambios estratégicos (re-diseño) en la sustentabilidad de esos predios, procurando compartir los resultados obtenidos con otros actores a nivel regional.
650 $aDESARROLLO SOSTENIBLE
650 $aINVESTIGACIÓN AGRARIA
650 $aPRODUCCION FAMILIAR
650 $aSOCIOLOGIA RURAL
700 1 $aALBICETTE, M.M.
700 1 $aRUGGIA, A.
700 1 $aSCARLATO, S.
700 1 $aBLUMETTO, O.
700 1 $aBORTAGARAY, I.
700 1 $aCARDOZO, G.
700 1 $aCASTAGNA, A.
700 1 $aCLARA, P.
700 1 $aGARCIA, F.
700 1 $aGILSANZ, J.C.
700 1 $aLEONI, C.
700 1 $aQUINTANS, G.
700 1 $aSCARLATO, M.
700 1 $aSILVERA, M.
700 1 $aTISCORNIA, G.
700 1 $aVILARO, F.
700 1 $aALBIN, A.
773 $tRevista INIA Uruguay, 2014, No.39, p.75-78.
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